each new assay. 1321N1, a human astrocytoma cell line. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. iD5. Find regional support. Increased fluorescence performance. MTT was purchased from Sigma-Aldrich (St. Fluorescent Polarization: Solid black plates, as above. Key features. It allows you to explore cellular pathways and protein activation and expression in one system. Promega. 58% for the detection module and 2. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Plate chamber design of the SpectraMax category 1 microplate readers. g. Introduction. Read height should be optimized for . The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. , 2014; Xiong et al. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Fluorescence Microplate Reader Comparison Chart. 2. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Then, the cell area was calculated for each. i3x. Multimode Reader Comparison Chart. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. We would like to show you a description here but the site won’t allow us. +44 118 944 8000. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Patented user-exchangeable cartridge design expands the system's detection capability making it. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. 314 low lamp level warning. Patented user-exchangeable cartridge design expands the system's detection capability making it. Supplier Page. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Detection of pro-inflammatory factors. Find regional support. Molecular Devicesの全商品を見る. The chamber. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 1. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. VAL014, right table) assays on the SpectraMax i3x reader are shown below. 2. ) deep, preferably with access on all sides. Contact Local Team. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). Home > Search Results > Danaher Inc > spectramax i3x. Eliminate time-dependent substrate addition steps. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. User-upgradeable detection modules allow you to expand the capabilities. Prime function 20 amol. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. With optimized reagents, option to operate the readers. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Description 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. SeeSpectraMax MiniMaxImagingCytometeronpage85. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. The dynamic range of the assay is about 3. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Figure 3. 001 0. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Automated for ease of use. The SoftMax Pro Software provides integrated instrument control. Example: [Drawer]. Robust,. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Introduction. Image analysis provides accurate data. SpectraMax i3x Multi-Mode Detection Platform User Guide. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Ferguson, Ph. *On the SpectraMax i3x reader only. Figure 4. See Unpacking the SpectraMax Paradigm Instrument on page 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. SpectraMax Quant dsDNA Assay Kits. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Use the 3. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 흡광 (Absorbance) 리더기. . The standard solutions (0. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Both Microplate Optimization and Read Height Adjustment were. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Nucleic Acid and Protein Quantitation. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. each new lot of plates, to ensure that the center of each well is read. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. 제품. 5 µL anti-cAMP-Cryptate. 4 / 5. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. We would like to show you a description here but the site won’t allow us. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. with best-in-class data analysis software • Three modes of detection for . *On the SpectraMax i3x reader only. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. 08, 0. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 1. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. . Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 304 signal level saturation. 04, 0. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. 0´ 104 4. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. SpectraMax i3x. 0´ 104 6. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. SpectraMax ® iD3 and iD5 Multi-Mode . Get a front row view of your cells. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 1. 4. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. 312 gain calibration failed. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Italic and boldface type are used for emphasis. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 313 reference gain check fail. Detection of pro-inflammatory factors. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Features. Eliminate time-dependent substrate addition steps. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 2 only) SpectraMax M-series Microplate readers. 4 mg mL −1; 1. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. Molecular Devicesの全商品を見る. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). com. Promega. 3: The cuvette chamber. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. Sunnyvale, CA, USA) with excitation at 475 nm. Increased fluorescence performance. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 10, and 0. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. QBT Fatty Acid Transporter Assay. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. The chamber. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Shake intensity. 蛍光 発光 ELISA. Ease of use. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. 200-800 nm using Spectra Max i3X microplate reader. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. 5 decades, with sensitive detection of cells. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. *On the SpectraMax i3x reader only. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Table 1. Description 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. Figure 4. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 価格、見積もり、お近くの販売店を. Features. Find regional support. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. SpectraMax Microplate Readers. Published Dec 16, 2020 | Updated Dec 16, 2020. Example: [Drawer]. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 2. Find regional support. , 2021). Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Multimode Reader Comparison Chart. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Lower limits of detection for ATP determined using the ATPlite . Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. , 2011). How to properly unlock iD series instrument. 3: The cuvette chamber. These concentrations are then used to perform western blots. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Enzyme Activation Analysis;. SpectraMax i3x Multi-Mode Detection Platform. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Increased fluorescence performance. Average 98 stars, based on 1 article reviews. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 01 0. Prismatic colour and motion for everyone. Microplate Reader. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The plate was shaken and incubated in the dark for 10 minutes. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. Bioz Stars score: 98/100, based on 1 PubMed citations. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. For wavelengths in the UV range above 220 nm,. 0001 0. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. 2. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 99 to $1999. Download PDF. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. SpectraMax i3x. Explore a wealth of applications with the future-ready SpectraMax i3x. Figure 1. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. It allows you to explore cellular pathways and protein activation and expression in one system. Order Now. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. com. In addition, Image J was used to quantify the. StainFree Cell Detection Algorithm. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Learn more about its features, specifications, and applications in this user guide. Controls containing all reaction components with either DI . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 7°C (gradient heating design). Find regional support. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Increased fluorescence performance. 00800 665 32860. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Structured Review. Carbonyl assay The plates for carbonyl detection were prepared as described. ) wide and 65 cm (25. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The plate was covered and incubated for one hour at room temperature. Find regional support. 8 Antibodies and. It also integrates with the. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Description 1. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Capture Flash Assays with Ease. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. D 1 1. We would like to show you a description here but the site won’t allow us. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. , 2011). HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). 635. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. SpectraMax i3x, onboard luminescence 121. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). 313 reference gain check fail. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Increased fluorescence performance. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. It allows you to explore cellular pathways and protein activation and expression in one system. Amplitude : 1. Wokingham, Berkshire RG41 5TS United Kingdom. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 315 can't find zero order. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. We would like to show you a description here but the site won’t allow us. Remove the transport bracket and store the retaining screws in the bag that held the 3. Bioz Stars score: 98/100, based on 1 PubMed citations. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Budget-friendly multi-mode microplate reader . The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. i3. The SpectraMax ® i3x reader provides the. The CellTiter-Glo assay from Promega uses luciferase. pathways and protein activation and expression in one system. Also refer to this Multimode Reader Comparison Chart. 4. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Nuclear Receptor. Description 1. i3x. . 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. These robust readers have been placed in labs from Antarctica to the. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Molecular Devices Corporation. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. MD Neurotransmitter Transporter Assay. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Cell counters for accurate and. 발광 (Luminescence) 리더기. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Instrument. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Multi-Mode Reader. XPS spectra were obtained from PHI Versaprobe II. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. no. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected.